ExFAB maintains a training lab available to students and faculty at Cal Poly Pomona, which provides hands-on training and access to specialized equipment.
ExFAB maintains a training lab available to students and faculty at Cal Poly Pomona, which provides hands-on training and access to specialized equipment including an Oxford Nanopore MinION Mk1D, a QuantStudio real-time PCR system, a Beckman Coulter Optima Max-XP benchtop ultracentrifuge, two New Brunswick Innova 42 high temperature incubator shakers, and an AKTA Pure chromatography system.
Capabilities include: sequencing of transcriptomes, bacterial and viral genomes/metagenomes, and 16S/18S rRNA genes; analysis of gene expression and nucleic acid abundance via quantitative PCR; isolation of viral particles, cellular organelles or subcellular fractions via high-speed centrifugation; purification of proteins, peptides and nucleic acids; and cultivation of thermophilic microbes.
If you are a Cal Poly Pomona researcher interested in using the ExFAB training lab, contact Jamie Snyder to discuss facility access and training.
Equipment List
Oxford Nanopore MinION Mk1D
Equipment Category: Nucleic Acids
Designed to connect with modern laptops via its USB-C port, it is compatible with MinION and Flongle Flow Cells. Allowing highly accurate real-time sequencing.
New Brunswick Innova 42 High Temperature Incubator Shakers
Equipment Category: Cell Culture
Static or shaking (19mm orbit, 25-400 RPM), with temperature control (ambient + 10 degrees to 95 degrees Celsius). 50ml - 2L volumes, up to 8L culture total. Continuous, timed, or multi-step programming.
Beckman Coulter Optima Max-XP benchtop Ultracentrifuge
Equipment Category: General
Separation of cellular organelles, membrane fractions, or viral particles. MLA-80 fixed angle rotor holds 6 x 175 µl tubes, max g-force of 440,000; TLA-55 fixed angle rotor holds 12 x 1.5 ml tubes, max g force of 186,000; MLS-50 swinging bucket rotor holds 4 x 5 ml tubes, max g force of 268,000. Temperature range: 0-40°C
Applied Biosystems QuantStudio 3 real-time PCR system
Equipment Category: Nucleic Acids
Quantitative PCR with up to 4 probes; 96 well (0.1 ml tubes) block with 3 independent temperature zones
Eppendorf 5415R Refrigerated Centrifuge
Equipment Category: General
Spinning down temperature-sensitive samples (e.g., DNA/RNA, proteins, cell cultures). Rotor holds 24 x 2 ml tubes; 16,000 xg max spin, Temperature range: 0-40°C
AKTA Pure chromatography system
Equipment Category: General
A versatile and modular system designed for reliable purification of biomolecules, including proteins, peptides and nucleic acids.
Questions? Contact us
Jamie Snyder
Co-Principal Investigator Cal Poly Pomona
jcsnyder@cpp.edu