ExFAB maintains a training lab available to students and faculty at Cal Poly Pomona, which provides hands-on training and access to specialized equipment.
ExFAB maintains a training lab available to students and faculty at Cal Poly Pomona, which provides hands-on training and access to specialized equipment including an Oxford Nanopore MinION Mk1D, a QuantStudio real-time PCR system, a Beckman Coulter Optima Max-XP benchtop ultracentrifuge, and two New Brunswick Innova 42 high temperature incubator shakers.
Capabilities include: sequencing of transcriptomes, bacterial and viral genomes/metagenomes, and 16S/18S rRNA genes; analysis of gene expression and nucleic acid abundance via quantitative PCR; isolation of viral particles, cellular organelles or subcellular fractions via high-speed centrifugation, and cultivation of thermophilic microbes.
If you are a Cal Poly Pomona researcher interested in using the ExFAB training lab, contact Kiya Kersh or Jamie Snyder to discuss facility access and training.
Equipment List
Oxford Nanopore MinION Mk1D
Equipment Category: Nucleic Acids
Designed to connect with modern laptops via its USB-C port, it is compatible with MinION and Flongle Flow Cells. Allowing highly accurate real-time sequencing.
New Brunswick Innova 42 High Temperature Incubator Shakers
Equipment Category: Cell Culture
Static or shaking (19mm orbit, 25-400 RPM), with temperature control (ambient + 10 degrees to 95 degrees Celsius). 50ml - 2L volumes, up to 8L culture total. Continuous, timed, or multi-step programming.
Beckman Coulter Optima Max-XP benchtop Ultracentrifuge
Equipment Category: General
Separation of cellular organelles, membrane fractions, or viral particles. MLA-80 fixed angle rotor holds 6 x 175 µl tubes, max g-force of 440,000; TLA-55 fixed angle rotor holds 12 x 1.5 ml tubes, max g force of 186,000; MLS-50 swinging bucket rotor holds 4 x 5 ml tubes, max g force of 268,000. Temperature range: 0-40°C
Applied Biosystems QuantStudio 3 real-time PCR system
Equipment Category: Nucleic Acids
Quantitative PCR with up to 4 probes; 96 well (0.1 ml tubes) block with 3 independent temperature zones
Eppendorf 5415R Refrigerated Centrifuge
Equipment Category: General
Spinning down temperature-sensitive samples (e.g., DNA/RNA, proteins, cell cultures). Rotor holds 24 x 2 ml tubes; 16,000 xg max spin, Temperature range: 0-40°C
AKTA Pure chromatography system
Equipment Category: General
A versatile and modular system designed for reliable purification of biomolecules, including proteins, peptides and nucleic acids.
Questions? Contact us
Jamie Snyder
Co-Principal Investigator Cal Poly Pomona
jcsnyder@cpp.edu
Kiya Kersh
Cal Poly Pomona ExFAB Lab Tech
kgkersh@cpp.edu