Automated QIAseq FX DNA Library Preparation on the Opentrons FLEX

DOI #10.17504 This workflow automates DNA-library construction for next generation Illumina short read sequencing by integrating the QIAseq FX DNA Library Kit with the Opentrons FLEX liquid-handling platform. A single FLEX script performs fragmentation, end-repair/A-tailing, adapter ligation, and bead clean-ups, eliminating manual pipetting and standardizing large-scale library preparation in 96-well plate format. The protocol yields consistent, high-quality libraries ready for short read sequencing while requiring very little hands-on time.